Roadmap Epigenome Project Protocols Database
Data Standards
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REMC Standards and Guidelines for ChIP-seq
v1.0
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REMC Standards and Guidelines for RNA-sequencing
DRAFT v3.0
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Standards and Guidelines for Whole Genome Shotgun Bisulfite Sequencing
Experimental Protocols
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5xMRE-seq library construction protocol v5
November 2010 -- Costello Lab
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ChIP Protocol for Full REMC (6 marks)
Broad
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Human hematopoietic cells: DNaseI treatment, crosslinking, and preserving cells for RNA
UW Stam Lab
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Isolation of modified histones from chromatin
Ren Lab
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Lymphoid cells from apheresis, Freezing
UW Stam Lab
Data Standards
Methylome Analysis
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Standards and Guidelines for Whole Genome Shotgun Bisulfite Sequencing
Dec. 2, 2011 —
ChIP-Seq
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REMC Standards and Guidelines for ChIP-seq
Dec. 2, 2011 — v1.0
RNA Analysis
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REMC Standards and Guidelines for RNA-sequencing
Dec. 2, 2011 — DRAFT v3.0