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Methylome Analysis
DNaseI
ChIP-Seq
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General Protocols
Data
Standards
REMC Standards and Guidelines for ChIP-seq
v1.0
REMC Standards and Guidelines for RNA-sequencing
DRAFT v3.0
Standards and Guidelines for Whole Genome Shotgun Bisulfite Sequencing
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Experimental
Protocols
5xMRE-seq library construction protocol v5
November 2010 -- Costello Lab
ChIP Protocol for Full REMC (6 marks)
Broad
Human hematopoietic cells: DNaseI treatment, crosslinking, and preserving cells for RNA
UW Stam Lab
Isolation of modified histones from chromatin
Ren Lab
Lymphoid cells from apheresis, Freezing
UW Stam Lab
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Methylome Analysis protocols
Standards and Guidelines for Whole Genome Shotgun Bisulfite Sequencing
Download this protocol here
5xMRE-seq library construction protocol v5
November 2010 -- Costello Lab
Download this protocol here
MeDIP-seq library construction protocol v2
June 2010 -- Costello Lab
Download this protocol here
MethylC-seq library preparation protocol
Ecker Lab
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Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling
Broad Institute
Triple-MRE-seq library construction protocol v5
March 2011 -- Costello Lab
Download this protocol here