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Roadmap Epigenome Project Protocols Database
Methylome Analysis
DNaseI
ChIP-Seq
RNA Analysis
General Protocols
Data
Standards
REMC Standards and Guidelines for ChIP-seq
v1.0
REMC Standards and Guidelines for RNA-sequencing
DRAFT v3.0
Standards and Guidelines for Whole Genome Shotgun Bisulfite Sequencing
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Experimental
Protocols
5xMRE-seq library construction protocol v5
November 2010 -- Costello Lab
ChIP Protocol for Full REMC (6 marks)
Broad
Human hematopoietic cells: DNaseI treatment, crosslinking, and preserving cells for RNA
UW Stam Lab
Isolation of modified histones from chromatin
Ren Lab
Lymphoid cells from apheresis, Freezing
UW Stam Lab
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RNA Analysis protocols
REMC Standards and Guidelines for RNA-sequencing
DRAFT v3.0
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SLX Transcriptome Library Construction Day 1
BC
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SLX Transcriptome Library Construction Day 2
BC
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SLX-micro RNA2
BC
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Total RNA Extraction from Cells UW-NREMC
UW Stam Lab
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Total RNA Extraction from Tissues UW-NREMC
UW Stam Lab
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